The general scopes of scientific interests:
The anti-tumor and anti-viral; osteogenesis-promoting; immunostimulating/immunomodulating activities of substances/compounds/extracts of natural, semi-synthetic, synthetic origin and molecular mechanisms of their activities - in vitro studies.
The biological evaluation of biomaterials - in vitro studies.
The main scientific interests:
The engagement of neutral endopeptidase (NEP, neprilysin, CD10) in biology of tumors, e.g. colon cancer and osteosarcoma.
The natural, semi-synthetic, synthetic compounds with anti-tumor potential and down-regulating or inhibiting activity of NEP.
Grants of National Science Center of Poland:
The role of neutral endopeptidase (NEP) and its inhibitor, sialorphin, in colon cancer cells biology, finished (supervisor Prof. dr hab. M. Kandefer-Szerszeń; editor, main executor dr M. Mizerska-Kowalska)
The evaluation of alfa-ketoglutarate (AKG) influence on level and activity of neutral endopeptidase (NEP) in tumor cells, finhished (editor, supervisor and main executor dr M. Mizerska-Kowalska)
Patents:
Lacasse enzyme isolated from Cerrena unicolor to be applied in the cervical carcinoma treatment. Patent no 40657313
Protein-sugar fraction isolated from the coelomic fluid of Dendrobaena veneta earthworm for application in non-specific immunotherapy. Patent no 238444
Patent applications:
[1-(N-p-bromophenylamino)]-1-(p-nitrophenyl)methylphosphonous acid-borane diisopropyl ester to be applied in the osteosarcoma treatment. Application no P.438017
[1-(N-p-bromophenylamino)]-1-(p-nitrophenyl)methylphosphonous acid-borane diisopropyl ester to be applied as inhibitor of neutral endopeptidase. Application no P.440633
Skills: colorimetric and fluorometric methods used for cell proliferation and viability evaluation; flow cytometry, RT-PCR, Western Blott, immunofluorescence and ELISA used for evaluation of expression level of extra- and intracellular proteins; wound closure and transwell-based assays to determine the metastatic activities of tumor cells, basic in silico analysis (ADME) on biologically active small molecular compounds; establishment of chicken fibroblast cell culture; viruses cultivation in cell cultures and chicken embryos; biological methods of evaluation of antiviral activity of any substances/compounds; biological evaluation of medical devices tests for in vitro cytotoxicity
